Scientific Glossary

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HPLC separates mixture components based on their differential affinity for a stationary phase vs. a mobile phase. For peptide purity analysis, reverse-phase HPLC (RP-HPLC) is standard. Results show a chromatogram with peaks representing different compounds — the target peptide peak area is compared to total peak area to express purity as a percentage. Pharmaceutical-grade peptides typically require >98% HPLC purity. Research-grade compounds often fall in the 95-99%+ range. HPLC data should be on any legitimate COA.